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. 2011 Oct 24;4:31. doi: 10.3389/fnmol.2011.00031

Figure 3.

Figure 3

Regulation of phospho-Ser-GSK3 by 5-HT2A receptor ligands in mouse brain. (A) Wild type mice were treated with saline (Veh) or the 5-HT2A receptor antagonist MDL11939 (MDL, 4 mg/kg, i.p., 1 h). Immunoblots of phospho-Ser21-GSK3α, phospho-Ser9-GSK3β, total GSK3α, and total GSK3β in the cerebral cortex, hippocampus, and striatum are quantified by densitometry. Mean ± SEM, *p < 0.05 when values are compared to saline treatment in Student’s t-test, n = 4–13. (B) Wild type (WT) and β-arrestin2-knockout (KO) mice were treated with saline (Veh) or DOI (20 mg/kg, i.p.) for 1 h. Immunoblots of phospho-Ser21-GSK3α, phospho-Ser9-GSK3β, total GSK3α, and total GSK3β in the cerebral cortex, hippocampus, and striatum are quantified by densitometry. Mean ± SEM, *p < 0.05 between saline and DOI treatment within the same genotype, Student’s t-test; **p < 0.05, two-way ANOVA followed by Holm–Sidak comparison, n = 10–12.