Figure 5.

N6L treatment results in a marked phenotypic change in human colon carcinoma cells giving typical circular colonies instead of spreading and growing in several layers. SW420 (in section A; phase contrast images) and SW620 (in section B, immunofluorescence images) cells were cultured in the presence of 10 μM N6L for 3 days before analysis (Methods). The immunofluorescence images show nucleolin in the nuclei of cells revealed with mAb D3. The images were taken at 100- and 1000-fold magnification in section A and B, respectively.