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. 2011 Aug 3;11:333. doi: 10.1186/1471-2407-11-333

Figure 6.

Figure 6

Selective down regulation of MMP-9 and nucleolin transcripts in Nucant treated SW620 cells. A. SW620 cells in the absence (lanes -) and presence (lanes +) of 10 μM of N7 were cultured for 24, 48, and 72 hours. B. Dose dependent reduction of MMP-9 and nucleolin transcripts in response to Nucant treatment. Newly seeded SW620 cells were cultured in the absence (lanes 1) and presence of 2.5 (lanes 2), 5 (lanes 3) and 10 (lanes 4) 10 μM of N6 for 20 hours. Total RNA extracts were prepared at different time points and the expression of various transcripts was analyzed by RT-PCR using specific primers (Methods). NCL stands for nucleolin; NPM stands for nucleophosmin.