Table 5.
Inhibitory activity of N3, N6, and N6L on melanoma TIII cell adhesion and proliferation.
| Nucant | % Inhibition of cell | |
|---|---|---|
| Adhesion | Proliferation | |
| N3: 5 μM | No effect | No effect |
| N3: 10 μM | 15% | 10% |
| N3: 20 μM | 24% | 36% |
| N6: 2.5 μM | 15% | 12% |
| N6: 5 μM | 26% | 20% |
| N6: 10 μM | 38% | 32% |
| N6: 20 μM | 55% | 45% |
| N6L: 2.5 μM | 39% | 45% |
| N6L: 5 μM | 49% | 56% |
| N6L: 10 μM | 65% | 80% |
| N6L: 20 μM | 72% | 85% |
Epithelial melanoma TIII cells were cultured in the absence or presence of different concentrations of N3, N6, and N6L to monitor inhibition of cell adhesion (after 5 hours of cell seeding) and cell proliferation (after 3 days of culture). The number of viable cells in untreated control samples was used to calculate % inhibition of cell adhesion and proliferation. The mean percentage values of at least 2 independent experiments are presented.