Table 6.
Nucant pseudopeptides inhibit proliferation of human colon carcinoma cells.
Cells | Nucant | Number of cells | % inhibition |
---|---|---|---|
SW480 | None: Control | 1,500,000 | - |
N7: 10 μM | 1,200,000 | 20% | |
N7: 20 μM | 900,000 | 40% | |
N6L: 10 μM | 800,000 | 47% | |
SW620 | None: Control | 1,800,000 | - |
N7: 10 μM | 1,000,000 | 44% | |
N7: 20 μM | 700,000 | 61% | |
N6L: 10 μM | 450,000 | 75% |
Cells seeded in 6-well plates at 20,000 cells/ml in 2 ml culture medium were cultured in the absence (Control) or presence of N7 and N6L. Seven days after seeding, cells were trypsinized and counted. Nucant pseudopeptides were added only once 5 hours after seeding of cells. Consistently, N6L manifests at least 2-fold higher inhibitory activity compared to N7.