Table 7.
IC50 values for the induction of cell death and reduction of cell number in T29 cells treated with HB-19 and related multivalent pseudopeptides.
| Pseudopeptide (Multivalent) | Cell death: IC50 24 hours post-passage | Cell number: IC50 72 hours post-passage |
|---|---|---|
| HB-19 (pentavalent) | > 20 μM | 20 μM |
| N3 (pentavalent) | > 20 μM | 5 μM |
| N6 (hexavalent) | 20 μM | 8 μM |
| N7 (hexavalent) | 20 μM | 8 μM |
| N6L (hexavalent) | 5-10 μM | 2-4 μM |
T29 cells were passaged in the presence of HB-19, N3, N6, N7, and N6L at various concentrations 0, 2.5, 5, 10 and 20 μM for 3 days. Twenty-four hours after the treatments, cell death was monitored by the uptake of trypan blue. The number of viable cells at 3 days post-treatment was used to estimate the IC50 values for the reduction of cell number. The pseudopeptides represent pentavalently or hexavalently the pseudo-tripeptide Lysψ(CH2N)-Pro-Arg (Figure 1).