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. Author manuscript; available in PMC: 2011 Oct 24.
Published in final edited form as: Biochemistry. 2010 Jul 27;49(29):6290–6295. doi: 10.1021/bi100932p

Figure 4.

Figure 4

Comparison of the effects of a protonated and deprotonated His side-chain on FCN fluorescence measured for the GGFCNXA peptides with an acetylated (blocked) N-terminus. Black, X=Ala at pH 5; red X=His at pH 5; blue, X=His at pH 10. All spectra were recorded in water at the indicated pH. Sample concentration was 20 uM and the excitation was at 240 nm.