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. Author manuscript; available in PMC: 2012 Feb 25.
Published in final edited form as: J Mol Biol. 2010 Dec 30;406(3):491–502. doi: 10.1016/j.jmb.2010.12.028

Figure 6. Fast green FCF inhibits amyloid formation by proIAPP1–48.

Figure 6

(a) The effects of fast green FCF on the kinetics of proIAPP1–48 monitored by thioflavin-T fluorescence: proIAPP1–48 alone (black); a mixture of fast green FCF and proIAPP1–48 at a ratio of 1:1 (red). (b) TEM image of proIAPP1–48 in the absence of inhibitor collected at 80 minutes after the initiation of the reaction. (c) TEM image of a mixture of fast green FCF and proIAPP1–48 at a 1:1 ratio collected at 80 minutes after the initiation of the reaction. (d) TEM image of a mixture of fast green FCF and proIAPP1–48 at a 10:1 ratio collected at 80 minutes after the initiation of the reaction. All samples were examined at pH 7.4. Scale bars represent 100 nm. Solutions contained 20 mM Tris-HCl buffer (pH 7.4), 25 µM thioflavin-T, 32 µM peptide, 2% HFIP, and were continually stirred at 25°C. The thioflavin-T curve shown and TEM images displayed for proIAPP1–48 without inhibitor are the same as shown in Figure 4.