SULT1E1 expression in preconfluent and confluent MCF10A cells. MCF10A cells were harvested at approximately 70 and 100% confluence. A, SULT1E1 mRNA levels were measured in six independent experiments using a TaqMan Gene Expression Assay. B, SULT1E1 immunoreactive protein levels were measured in two independent experiments by Western blot hybridization. C, SULT1E1 hnRNA levels were measured in six independent experiments using a SYBR Green Real-Time RT-PCR assay. In A and C, data are expressed as means ± S.E.M. ***, p < 0.001 compared with preconfluent cells.