Fig. 5.
The increase in ethanol sensitivity produced by activated Gαs depends on βγ subunits. A, whole-cell recordings obtained in HEK293 cells that coexpressed Gαs Q-L and the Gβγ sequester ct-GRK2. Current traces during the application of 1 and 100 mM ethanol are shown. B, concentration response curves for ethanol (1–200 mM) show that the shift in the ethanol sensitivity promoted by Gαs Q-L (■) is blocked by expression of the Gβγ sequester (●). Differences were significant (***, P < 0.001, ANOVA). C, traces are Cl− currents induced in two α1 GlyRs with intracellular mutations in cells overexpressing Gαs Q-L during the application of different ethanol concentrations. D, graph shows that the expression of the constitutively active Gαs did not modify the ethanol sensitivity of either 316–320A (■) or 385–386A (●) mutated GlyRs with respect to cells expressing Gαs Q-L (○ and □). Differences were not significant.