Fig. 6.
Effect of acute perinatal exposure to THC on the immune response of postnatal 5 weeks of age to gp120. Groups of two C57BL/6 pregnant mice (n = 2) were injected with 50 mg/kg THC or vehicle on GD16. Five weeks after the pups were born, they were injected in each rear footpad with 5 μg of HIV-1 p17/p24/gp120 emulsified in CFA. After 1 week, sera (A) and draining LNs (B) were collected. A, sera were analyzed for the presence of HIV-1 p27/p24/gp120-specific IgG by ELISA. B, the draining LN cells were left unstimulated or restimulated with 25 μg/ml of HIV-1 p17/p24/gp120 for 72 h. During the final 8 h, the cells were pulsed with 2 μCi of [3H]thymidine. Thymidine incorporation was determined by β-scintillation counting. The data represent mean ± S.E.M. of triplicate cultures. *, statistically significant differences, p ≤ 0.05. A, one-way ANOVA, interaction, p = 0.0005; DMSO vehicle versus THC, p = 0.017; untreated versus gp120, p < 0.0001. B, one-way ANOVA, interaction not significant; DMSO versus THC, not significant; untreated versus gp120, p = 0.0016.