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. 2011 Nov;339(2):394–402. doi: 10.1124/jpet.111.183400

Fig. 4.

Fig. 4.

VSMC migration assays. A to C, representative photomicrographs of scrape-injured confluent cells showing borders of denuded regions. A, vehicle-treated control at time 0. B, vehicle-treated cells after 18 h. C, BAY (10 μM)-treated cells after 18 h. BAY significantly reduced 10% FBS-stimulated VSMC migration after scrape injury after 18 h at both 0.1 μM (50%) and 10 μM (60%). D, data showing inhibition of VSMC migration by BAY after scrape injury and stimulation by 10% FBS. Percentage of regrowth after injury was calculated after normalizing readouts to time 0 controls. E, migration was measured using the transwell method with PDGF (20 ng/ml) stimulation after 6 h. BAY significantly reduced (∼50%) chemotaxis after 6 h compared with vehicle controls. Data are mean ± S.E.M., with n = 3 for both assays, each performed in duplicate. **, p < 0.01; ***, p < 0.001, Student-Newman-Keuls method for multiple comparisons after one-way ANOVA was used.