Fig. 6.

The presence of BAY (10 μM, 60 min) stimulated phosphorylation of VASP at Ser₁₅₇ and Ser₂₃₉ detected by In-Cell and traditional Western blotting. A, the stimulation at pVASPSer₁₅₇ is doubled (p < 0.001) compared with vehicle controls. B, stimulation of pVASPSer₂₃₉ is tripled (p < 0.001) compared with vehicle controls. Data are mean ± S.E.M. of three independent experiments each performed in triplicate. ***, p < 0.001. C and D, representative images of Western blotting showing concentration-dependent increases in VASP phosphorylation at both Ser₁₅₇ and Ser₂₃₉ after BAY treatment (0.1–10 μM; 24 h) compared with the vehicle controls (C) and increases in total VASP content (VASP_Tot) after BAY treatment (0.1–10 μM; 24 h) (D).