Fig. 7.

Site-specific VASP phosphorylation by BAY: roles of PKA versus PKG signaling. A, using pVASPSer₁₅₇ as a readout for PKA activity, increased expression of pVASPSer₁₅₇ by BAY is further enhanced with the PKA inhibitor PKI, whereas the PKG inhibitor DT-2 failed to exert noticeable changes. B, using pVASPSer₂₃₉ as readout for PKG activity, inhibition of PKG by DT-2 completely reversed BAY stimulation of pVASPSer₂₃₉, but PKA inhibition by PKI again further increased the levels of pVASPSer₂₃₉ compared with BAY alone. Data are mean ± S.E.M. n = 3 per treatment group. *, p < 0.05; Student-Newman-Keuls method for multiple comparisons after one-way ANOVA.