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. 2011 Nov;339(2):487–498. doi: 10.1124/jpet.111.184341

Fig. 7.

Fig. 7.

Suppression of autophagy enhances fatty acid-induced cell death and lipid accumulation. A, HepG2 cells were treated with vehicle control (5% BSA), OA (500 μM), OA plus CQ (20 μM), CQ alone, OA plus 3-MA (10 mM), or 3-MA alone for 24 h, and apoptotic cell death was analyzed by nuclear staining with Hoechst 33342 (mean ± S.E., n = 3). #, p < 0.01 (one-way ANOVA with the Scheffé post hoc test). B and C, HepG2 cells were treated with vehicle control (5% BSA) or various concentrations (125, 250, or 500) of OA or PA for 6 h (B) or with OA (500 μM) or PA (500 μM) in the presence or absence of CQ (20 μM) for 6 h (C). Total cell lysates were subjected to immunoblot analysis for perilipin A. Densitometry analysis for the expression level of perilipin was performed using ImageJ software, which was further normalized with its loading control (β-actin). D, HepG2 cells were treated as in C, and cellular TG levels (mean ± S.E., n = 3) were quantified as described under Materials and Methods. #, p < 0.01 (one-way ANOVA with the Scheffé post hoc test).