Fig. 8.

A high-fat diet induces steatosis and autophagy in mouse liver. Male C57BL/6J mice were fed either a control diet or a Western diet for 3 months. All the mice were starved for 16 h before they were sacrificed. A, representative photomicrograph of hematoxylin and eosin-stained liver section from a mouse fed a control diet (a) and from a mouse fed the high-fat diet (b). c, enlarged photomicrograph from b showing typical macrovesicular hepatic steatosis (arrows). B, liver samples were processed for EM. a, control diet. b and c, high-fat diet. d and e, enlarged photomicrographs from the boxed areas in c. Arrows, double membrane autophagosomes; N, nuclei, LD, lipid droplets; M, mitochondria. C, total liver lysates were subjected to Western blot analysis using an anti-LC3 antibody. The same membrane was blotted for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the loading control. D, densitometry analysis for the expression level of LC3-II was performed using ImageJ software, which was further normalized with its loading control (GAPDH). Data are presented as the fold of the control diet mouse livers (mean ± S.E., n = 6). #, p < 0.01, Student's t test.