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. 2011 Nov;339(2):618–623. doi: 10.1124/jpet.111.185876

Fig. 8.

Fig. 8.

A, structure of the two-site ELISA for detection of antibodies against the cTfRMAb-TNFR fusion protein. The cTfRMAb-TNFR fusion protein is used as the capture reagent, and the biotinylated cTfRMAb-TNFR fusion protein is used as the detector reagent, along with a complex of streptavidin (SA) and horseradish peroxidase (HRP); the biotin moiety is designated, B. B, Western blot of nonbiotinylated cTfRMAb-TNFR fusion protein (lane 1) and biotinylated cTfRMAb-TNFR fusion protein (lane 2) with a conjugate of avidin and biotinylated peroxidase. C, absorbance at 1:50 dilutions of mouse plasma taken preinjection or postinjection after 3 weeks of either intravenous injections with either etanercept or the cTfRMAb-TNFR fusion protein. Data are shown for all 10 mice in each group.