Table 1.
Synthetic arrays generated from different types of repeats
| Repeat unit | Size of unit | Size of array | Fold increase |
|---|---|---|---|
| Human alphoid DNA24-28 | |||
| 2 mer | 0.34 kb | 40 kb | x118 |
| 2 mer/mutant* | 0.34 kb | 60 kb | x176 |
| 4 mer | 0.68 kb | 70 kb | x103 |
| 5 mer | 0.85 kb | 140 kb | x165 |
| 6 mer | 1.02 kb | 35 kb | x35 |
| tetO-CENP-B/2 mer** | 0.34 kb | 50 kb | x147 |
| tetO-CENP-B/2 mer** | 0.34 kb | 50 kb | x147 |
| Human gamma-satellite DNA29 | |||
| 1 mer | 0.24 kb | 24 kb | x100 |
| Mouse satellite DNA29 | |||
| Major, 3 mer | 0.7 kb | 55 kb | x79 |
| Minor, 4 mer | 0.5 kb | 10 kb | x20 |
*Dimer from a chromosome 17 alphoid 16-mer higher order repeat containing a mutated CENP-B biding motif (CENP-B box).
**Novel artificial alphoid dimers composed of one monomer from a chromosome 17 alphoid 16-mer higher order repeat linked to a wholly synthetic alphoid monomer based on a published consensus sequence. The natural monomers contain either a wild type or mutated CENP-B box. In the synthetic monomer, this was replaced with a 42 bp tetracycline operator (tetO), the binding site for E. coli tetracycline repressor (tetR).