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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Dev Biol. 2010 Oct 8;348(2):199–209. doi: 10.1016/j.ydbio.2010.09.024

Figure 6.

Figure 6

Activation of Hh signaling after the closure of RP resulted in increased proliferation in the anterior-dorsal pituitary. (A-C) X-gal staining revealed the activity of Pitx2Cre from E9.5 to E12.5. (D-I) Analysis of patterning markers in E12.5 WT embryos (D,F,H) and in embryos with activated Hh signaling (Pitx2Cre;SmoM2) using RNA in situ hybridization (D-F,H-J) and immunostaining (G, K). (L, M) Immunostaining of BrdU incorporation and the expression of Ki67. (N) Quantification of BrdU incorporation and Ki67 expression. (* indicates P<0.05, n=6 embryos). (O-V) Activation of Hh signaling did not affect pituitary patterning, as revealed by RNA in situ hybridization of markers at E14.5. Scale bars: 100 μm.