Table III.
Inoculated Virus Strain | CP Sequence Identitya | Plants with Symptoms
|
|
---|---|---|---|
Transgenicb | Nontransgenic control | ||
% | no. | ||
SrMV-SCH | 100 | 3/25 | 8/10 |
SrMV-SCM | 95 | 8/25 | 5/5 |
SrMV-SCI | 95 | 7/25 | 3/3 |
SCMV-D | 75 | 22/25 | 5/5 |
Nucleotide sequence identity between the CP ORF of the transgene and the inoculated virus strain.
SrMV-SCH-resistant transformants from group 4 were vegetatively propagated by cuttings and shoots were sap inoculated with different SrMV and SCMV strains at the three- to five-leaf stage. Symptoms were visually scored 2 months after infection.