Table 1.
Parent Set of Peptides and Sets 1 and 2 of Mutants. (Unless indicated, N- and C-termini are free.)
| Peptide | Charge (pH 7.5) | Length (residues) | μHa | Sequence |
|---|---|---|---|---|
| Set of Parent Peptides
| ||||
| δ-Lysin | 0 | 26 | 7.8 | formyl-MAQDIISTIGDLVKWIIDTVNKFTKK |
| Cecropin A | +7 | 37 | 4.9 | KWKLFKKIEKVGQNIRDGIIKAGPAVAVVGQATQIAK-amide |
| Magainin-2 F12W | +3 | 23 | 6.9 | GIGKFLHSAKKWGKAFVGEIMNS |
|
| ||||
| Set 1 of Mutant Peptides
| ||||
| DL-1 | +6 | 26 | 7.2 | formyl-MAQKIISTIGKLVKWIIKTVNKFTKK |
| CE-1 | +1 | 37 | 7.3 | EWKLFEKIEKLGQNILDGIIKLGPLLALLGQLTQIAL-amide |
| MG-1 | 0 | 23 | 9.1 | GILKFLESAKKWLEAFLAEIMNS |
|
| ||||
| Set 2 of Mutant Peptides
| ||||
| DL-2a | 0 | 26 | 7.7 | formyl-LAADLLAALGDLAKWLLDALAKAAKK |
| DL-2b | 0 | 26 | 8.8 | formyl-LAADLLAALGDLLKWLLDALAKLAKK |
| CE-2 | +7 | 37 | 5.2 | KWKLLKKLEKAGAALKEGLLKAGPALALLGAAAALAK-amide |
| MG-2 | +3 | 23 | 7.0 | GLGKLLHAAKKLGKAWLGELLAA |
a
Hydrophobic moment of the complete helix calculated with MPEx using the Wimley-White interfacial scale (18).