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. 2011 Oct 24;6(10):e25854. doi: 10.1371/journal.pone.0025854

Figure 3. Western Blot analyses of constructs used for cell transfection.

Figure 3

The integrity of the constructs used to transfect cells (shown in Figure 2) was assessed by Western Blotting. After separation of the proteins in cell lysates by SDS-PAGE, the bands were transferred to nitrocellulose membranes and for detection with anti-EGFP and anti-tubulin antibodies followed by peroxidase-labeled anti-rabbit IgG. The protein bands were detected by chemiluminescence. 1. Lysed Huh 7 cells; 2-Huh 7 cells transfected with pEGFPN1, 3-Huh7 cells transfected with core sequence aa(1-173) inserted into pEGFPN1; 4-Huh 7 cells transfected with the core aa(1–160) sequence in pEGFPN1; Huh 7 cells transfected with core sequence aa(1–140) inserted into pEGFPN1.