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. 2011 Oct 24;6(10):e25854. doi: 10.1371/journal.pone.0025854

Figure 8. Nuclear export of core NES is mediated by CRM-1.

Figure 8

Huh7 cells were transfected with a plasmid encoding EGFP-tagged NLS SV40 (a, b), EGFP-tagged NLS SV40 and HIV Rev NES (c, d) or the plasmid encoding EGFP-tagged NLS SV40 and putative core NES, aa(109-133) (e, f). Cells transfected with the EGFP-tagged control plasmid pEGFPC1 are shown (g, h). Twenty-two h after transfection, the cells were treated for 4 h with 10 ng/ml LMB (b, d, f, h; +LMB). The cells were subsequently washed, fixed and analyzed by immunofluorescence microscopy. The bar represents 10 µm.