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. 2011 Oct 24;6(10):e25854. doi: 10.1371/journal.pone.0025854

Figure 9. Subcellular localization of core in LMB-treated cells.

Figure 9

(A) Production of the EGFP-labeled core protein aa(1–173) in Huh7 cells results in the presence of this protein in both the cytoplasmic (a) and nuclear (b) compartments. Huh7 cells were transfected with a plasmid encoding EGFP–labeled aa(1–173) protein and, 40 h later, cells were treated with 10 ng/ml LMB. The inhibition of nuclear export by LMB induced an increase in the accumulation of HCV core protein in the nucleus (c). (B) Subcellular localization of core protein, with and without LMB treatement, confirmed by confocal microscopy analyses. Huh7 cells were transfected with a plasmid encoding the EGFP-labeled core protein aa(1–173), as described in (A). Slides correspond to the panels shown in A: the cytoplasmic (a) and nuclear (b) distribution of the core protein in non treated cells and its nuclear localization after LMB treatment (c). The bar indicates 20 µm.