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. 2011 Oct 24;6(10):e26381. doi: 10.1371/journal.pone.0026381

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Long, Rossi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–C) The total number of myotubes (white bars) as well as the number of GFP-positive myotubes (green bars) was determined by visual inspection of randomly selected, low power (5x) fields at 1 day (A), 2 days (B) and 3 days (C) post-fusion. The number of GFP-positive myotubes observed following Hα7-mediated fusion was significantly higher on all days than the number observed following PEG-mediated fusion (green asterisks). The total number of myotubes surviving PEG treatment was significantly lower than the total number observed in co-cultures lacking any fusogen (black asterisks). ***: p<0.001 (unpaired t-test). Data are shown as mean ± s.d. of three independent fusion experiments.