Skip to main content
. 2011 Oct 24;6(10):e26582. doi: 10.1371/journal.pone.0026582

Figure 4. Expression of glucocorticoid, mineralocorticoid and thyroid hormone receptors in the lateral and basolateral nuclei of the amygdala in euthyroid and hypothyroid rats.

Figure 4

(A) Amygdala volume estimation in E and H rats. No differences were found between groups. Representative photomicrographs (40×) of MR (B, C) and GR (E, F) immunohistochemistry in LA/BLA in E (Eu) and H (Hypo) animals. Scale bar, 20 µm. Histograms show the mean gray values of GR (D) and MR (G) obtained for E and H animals (n = 4 per group). GR and MR staining were increased in H rats. Panoramic views of isotopic in situ hybridization for TRα1 (H, I) and TRβ1 (K, L) in coronal sections of the brain of E (Eu) and H (Hypo) animals. Scale bar, 0.15 cm. Arrows point to the amygdala. Histograms show the mean gray values in arbitrary units (AU) obtained for E and H rats for TRα1 (J) and TRβ1 (M) mRNA expression (n = 3 per group). No differences were found between groups for TRα1. TRβ1 expression was reduced in H rats. Plot bars show means ± SEM. *, p<0.05; **, p<0.01; NS, no significant.