Figure 5. RNA interference of Notch2 augments phenethyl isothiocyanate (PEITC)-mediated inhibition of LNCaP and PC-3 cell migration.

Representative images (Boyden chamber assay) depicting migration of (A) LNCaP and (C) PC-3 cells transfected with a control (non-specific) siRNA or a Notch2-targeted siRNA and treated for 24 hours with dimethyl sulfoxide (DMSO) or 5 µM PEITC at 200× magnification. Quantitation of migrated (B) LNCaP cells and (D) PC-3 cells from experiments shown in panels A and C. Three to four fields on each filter were scored for migrated cells under an inverted microscope at 200× magnification. Results shown are mean ± SD (n = 3). *Significantly different (P<0.05) between the indicated groups by one-way ANOVA followed by Bonferroni's multiple comparison test. Each experiment was repeated twice, and representative data from one such experiment are shown.