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. 2011 Oct 24;6(10):e26687. doi: 10.1371/journal.pone.0026687

Table 1. Primer sequences used for quantitative PCR for both A. aspera and Symbiodinium clade C1.

Organism Gene name Forward Primer Reverse Primer Reaction efficiency Accession number or citation
Coral Glutamine synthetase TTTGGATCGATGGAACAGGAGAG TCGGCACTAAGAGGTTCGGATTC 1.10 DY579366.1
HSP90 TTTCTTGTTGCTGACCGTGTAATAGTA CCCCTGGGATCCTCTGTGA 0.99 DY584045.1
HSP70 GTCGCTCTCAATCCATCAAATACT GTCTCCCACCTTCGCTTACG 0.98 GO000475.1
Malonyl Co-A carboxylase GTTGGGCGGGGACACATCATC GCAGACGTCATCAGTTCAGTAT 1.02 EZ001045.1
α-ketoglutarate dehydrogenase AGTTAGGCTTCATGTCCGCTTTCA GACAATCTCACTGCCTTCAATAACTGC 1.00 EZ012855.1
Glycogen phosphorylase CTCAGGAAAGCCATCAATCAAATCAGG TGCATCAAAGTCGGCCATAAGAAGG 1.01 EZ031953.1
Glycogen synthase TCAAATCTGCCGAGGAATCAATCAA AGTCGCTCCGTTCTGTTTCTCTGG 1.01 GW213759.1
GAPDH GAGGCTGGTGCAGATTTTGT TGACTTTCTTGGCTCCACCT 1.04 EZ026309.1
rpS7 AGCAAAGGAGGTTGATGTGG GACGGGTCTGGATCTTTTGA 0.97 [59]
AdoHcyase AAGAAGACAAACATCAAGCCTCA CACATCCAAGGTTCACAAGACG 0.94 [59]
Symbiodinium Glutamine synthetase GAGGAAACCGAGGCCCGTCAGGAAGTGA AGCAGGCAAGAAGCCGGTCTCGGTCATC 1.00 JF915366
HSP90 GCTTGAGTTCCGTGCTTTGTTGT CTTCGCACGTAGAGTTTGATGTTGT 1.04 EH038163.1
HSP70 TTTGAGGAGCTGTGCATGGACTACTT GGAACCACCCACCAGAACCACATCAT 1.03 EH037708.1
Malonyl Co-A carboxylase TTTCCAAAGGGCTTCTCGTGTGC ACCCTTCTTCTCAGCCAGCTCCTTCAG 0.99 JF915367
α-ketoglutarate dehydrogenase ATCAAGATGCGAAAGGAATACAAG GCATAAGCACTGGCCAGAAAGAAC 0.99 EH037114.1
GAPDH GCCCTGCGTGTGCCAACCAT CTTCATCTCAGCGCAAATCTCCTCGTA EH036392.1
β-actin TGGACAACGGAAGCGGAATG GCCAACAATGGATGGGAAAACT 1.04 [24]
PCNA GAGTTTCAGAAGATTTGCCGAGAT ACATTGCCACTGCCGAGGTC 0.98 [24]