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. 2010 Nov 3;30(44):14805–14816. doi: 10.1523/JNEUROSCI.1161-10.2010

Figure 2.

Figure 2.

Broad distribution and synaptic enrichment of IB2 protein. A, Immunoblot detection of IB2 in mouse brain. Triton X-100-soluble extracts from whole brain or brain regions of IB2+/+ and IB2−/− mice were electrophoresed and immunoblotted, probing with monoclonal antibody to IB2. IB2 protein running with apparent molecular weight of 150,000 is expressed in all examined regions. B, IB2 enrichment in postsynaptic densities. Homogenates from IB2+/+ and IB2−/− cerebral cortex (left) or cerebellum (right) were used to prepare synaptosomes (SYN) or glomeruli (GLO), respectively, each of which was then used to extract PSDs. Equal amounts of protein from all fractions were analyzed by immunoblotting for IB2, PSD-95, and synaptophysin (SYP). PSDs from IB2+/+ cortical synaptosomes and cerebellar glomeruli are enriched for IB2 and PSD-95. C, Coimmunoprecipitation of IB2 and other PSD proteins. Whole-brain Triton X-100-soluble extracts from IB2+/+ and IB2−/− mice were immunoprecipitated with anti-IB2 and then immunoblotted with antibodies to IB2, PSD-95, AMPAR subunit GluR2, or NMDAR subunit NR2B. All of these PSD proteins coimmunoprecipitated with IB2 from the IB2+/+ extract.