Figure 1.

ERD1 protein is down-regulated during senescence, whereas its mRNA is up-regulated. A, RNA and protein blots derived from the fifth true leaf of wild-type Arabidopsis (ecotype Ler) probed with ERD1 polyclonal antibodies or labeled cDNA (left) or with c-myc monoclonal antibodies or labeled DNA (right). B, RNA and protein blots derived from the fifth true leaf of Arabidopsis 15myc Ler, which is Ler transformed with a c-myc-tagged ERD1 genomic clone, and probed as in A. Young leaves averaged 15 mm and were harvested 16 d after germination; early fully expanded (exp) leaves averaged 27 mm and were harvested 20 d after germination; late fully expanded leaves averaged 30 mm and were harvested 23 d after germination; early senescent (sen) leaves averaged 15% yellow and were harvested 29 d after germination; late senescent leaves averaged 70% yellow and were harvested 33 d after germination. RNA and protein derived from equal volumes of leaf tissue were loaded.