Figure 1.

Correlation between ER morphology, IP3R clustering, and IP3R Ca²⁺ release plasma. (A) In Non-CF cells, the ER was expended at the totality of cell surface, IP3Rs are distant between others and the IP3R ER Ca²⁺ release was normal (B) In CF cells, the F508del-CFTR was trapped into ER. The ER was concentrated around the nucleus. IP3Rs are more clustered and the Ca²⁺ propagation wave was abnormally increased. ER staining was performed with ER-tracker (1 μM during 15 min). The IP3R Ca²⁺ release was measured by using NP-EGTA or IP3-caged techniques.