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TUNEL assay testing activity of PTX-DNA@AuNPs 3 in MCF7 (A) and MES-SA/Dx5 (B) cells. TUNEL (green) and counter-stain (red) images of cells left untreated, DNA-AuNPs, 100 nM of free Paclitaxel and compound 1, and PTX-DNA@AuNPs 3 at the equivalent Paclitaxel concentrations of 50 nM and 100 nM. Scale bars are 50 µm. After treatment by PTX-DNA@AuNPs 3 for 48 h, significant numbers of TUNEL-positive cells and reduced populations are observed in both tested cell lines.
