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Feeding of cyclo-Dopa to five 23-d-old de-rooted fodder beet plants via hypocotyls for 1.5 h and betalain analysis of hypocotyl extracts by HPLC. A, Control (80 mm ascorbate); B, cyclo-Dopa feeding (2 mm, in the presence of 80 mm ascorbate). Scales of A405 (full scale = 0.12 absorbance units) and A540 (full scale = 0.5 absorbance units) are the same in A and B. Peak numbers correspond to the biosynthetic scheme in Figure 10. 3′, Isobetanidin (the [2S/15R]-isomer of betanidin).
