Table III.

Feeding of amino acids to hairy root cultures of yellow beet, line 5A

Amino Acid (2 mm)			Constitutive Betaxanthins
	Formed Betaxanthins		Miraxanthin V		Betalamic acid
	nmol/HRC	% Incorporation	nmol/HRC	% Control	nmol/HRC	% Control
d-7 Extract	–	–	998	–	327	–
Control	–	–	1457	100	345	100
Gly	98	0.15	1560	107	328	95
(S)-Ala	87	0.14	2477	170	431	124
(S)-Ser	210	0.34	2188	150	229	87
(S)-Thr	497	0.80	1471	101	209	61
d-7 Extract	–	–	1015	–	311	–
Control	–	–	2297	100	621	100
(S)-Leu	946	1.53	1543	67	265	43
(S)-Ile	603	0.97	1373	60	264	43
(S)-Val	426	0.69	1857	81	267	43
d-7 Extract	–	–	761	–	290	–
Control	–	–	1417	100	372	100
(S)-Gln	197	0.32	1672	118	343	92
(S)-Asn	162	0.26	1520	107	243	65
(S)-Glua	35	0.06	2448	172	329	88
(S)-Asp	28	0.05	1812	128	260	70
d-7 Extract	–	–	1289	–	312	–
Control	–	–	2271	100	319	100
(S)-Lys	128	0.21	2890	127	192	69
(S)-Arg	285	0.46	2184	96	142	45
(S)-Orn	84	0.14	1967	87	86	27
(S)-Met	479	0.77	1775	78	98	31
(S)-Trp	245	0.40	1792	79	92	29
d-7 Extract	–	–	896	–	446	–
Control	–	–	2958	100	272	100
(S)-Phe	646	1.04	1423	48	109	24
(S)-His	1204	1.94	2375	80	96	22
(S)-Pro	300	0.84	3127	106	255	57
(S)-Hyp	877	1.41	2184	74	93	21
Control	–	–	2326	100	520	100
(S)-ThiaPro	701	1.13	1192	51	465	89

Amino acids were fed by sterile filtration to hairy root cultures (HRC) (final concentration, 2 mm) at d 7 after subcultivation. After 24 h the hairy roots were harvested and analyzed as detailed in Methods. Values are means of two independent experiments.

^a(S)-Gln-betaxanthin (vulgaxanthin I) was formed.