Table I.
Reaction | Interaction Sites
|
||||
---|---|---|---|---|---|
Pallasa | BCPMla12 | BCPMlg | BCPmlo5 | DCINA-Induced | |
h | % | ||||
Haustorium/elongated secondary hypha | |||||
22 | 58 | 62 | 2 | 0 | 29 |
30 | 65 | 54 | 1 | 0 | 35 |
48 | 68 | 5 | 1 | 0 | 26 |
Effective papillab | |||||
22 | 32 | 21 | 58 | 99 | 40 |
30 | 17 | 18 | 23 | 99 | 22 |
48 | 22 | 10 | 21 | 98 | 32 |
HR (epidermis)c | |||||
22 | 10 | 17 | 40 | 1 | 31 |
30 | 18 | 28 | 76 | 1 | 43 |
48 | 10 | 64 | 78 | 2 | 42 |
HR (mesophyll) | |||||
22 | 0 | 0 | 0 | 0 | 0 |
30 | 0 | 0 | 0 | 0 | 0 |
48 | 0 | 21 | 0 | 0 | 0 |
Interaction phenotypes were microscopically analyzed at 22, 30, and 48 h after inoculation. The data represent each 100 interaction sites per leaf. Each of three independent experiments gave very similar results.
Results shown are from a plant treated with wettable powder as a control for plants treated with DCINA. Wettable powder did not affect the interaction (not shown).
Effective papilla in dead cells were evaluated as HR (epidermis).
In BCPMla12, HR occurred after penetration, whereas in all other NILs, dead cells did not contain a haustorium.