Figure 1.

(A) Example of a 4-parametric fitted standard curve in a competitive ELISA setting with peptide concentrations of 0, 3.125, 6.25, 12.5, 25, 50, 100 and 200 ng/ml. (B) Competitive ELISA test of MMCN-151 antibody reaction towards free peptide, elongated peptide, mimecan cleaved with MMP12 and intact mimecan. The x-axis is representative of the concentrations of free and elongated peptide. Concentrations of samples containing mimecan and mimecan cleaved with MMP12 are 43 ug/ml as the highest concentration followed by 2-fold dilutions of samples.