Figure 2.

Hypoxia inducible factor-1α expression is crucial in the hypoxic downregulation of ciliogenesis. MSC were transiently transfected with a scrambled siRNA sequence (Mock) or an siRNA designed to downregulate HIF-1α (siHIF-1α) as described in the Methods section. (A) Cells were then cultured either under normoxic or hypoxic conditions for 24 hours and cilia staining was performed. (B) Quantification of ciliogenesis in MSC cultured under normoxic (open bars) or hypoxic (black bars) conditions. Data are representative of three independent experiments. Probability values of less than 0.05 were considered significant, and an asterisk (*) identifies such significance relative to the respective control treatment.