Figure 3.

HIF-1α overexpression dowregulates ciliogenesis. MSC stably expressing a ΔODD HIF-1α mutant (MSC-HIF) were generated as described in the Methods section. (A) Cells were then cultured under normoxic conditions for 24 hours and cilia staining performed. (B) Quantification of ciliogenesis in MSC (open bars) or MSC-HIF (black bars) was performed. Data are representative of three independent cell cultures. Probability values of less than 0.05 were considered significant, and an asterisk (*) identifies such significance between MSC and MSC-HIF.