Figure 6.

Overexpression of sFRP-2 or gene silencing of sFRP-1, -3, -4 family members dowregulates ciliogenesis. (A) Gene silencing was performed using specific siRNA for each of the sFRP-1, -2, -3, or -4 members as described in the Methods section. Total RNA was extracted and qRT-PCR performed in order to evaluate efficiency and validate gene expression. (B) Cells were transfected with Mock or sFRP-2 cDNA plasmid, and sFRP-2 gene expression validated by qRT-PCR. (C) Primary cilium staining was performed in Mock- (white bars) and in sFRP-2- (black bars) transfected cells. (D) Gene silencing was performed with a scrambled sequence (siScr; Mock), or with the respective sFRP-1, -2, -3, -4 siRNA sequences. Primary cilium staining was performed in Mock- (white bars) and in si-sFRP-transfected cells (black bars).