Table I.

Identification of GAs from L. temulentum leaves by GC-MS

Putative and Ref GA	HPLC Fraction		KRI	GA Library Matcha	Multiple Ion Monitoring Results
	C18	N(CH3)2
					% of M+ ion area
					594(M+)	579	535	504	448	379	281	238	207
Lt GA8	8–11	29–31	2685	93	100	4	3	1	12	5	3	6	13
GA8 std	–	–	2685	93	100	5	4	1	14	7	3	6	10
					506(M+)	491	447	389	375	303	235	207
Lt GA29	8–11	39–42	2586	91	100	8	6	5	7	13	4	21
GA29 std	–	–	2586	91	100	10	5	6	8	15	3	14
					506(M+)	491	448	376	375	357	313	235	207
Lt GA1	14–17	33–35	2585	78	100	7	12	10	6	2	4	4	11
GA1 std	–	–	2586	95	100	8	14	9	6	2	4	3	13
					504(M+)	489	475	460	445	431	387	370	311	238	208
Lt GA3	14–17	42–44	2612	86	100	6	4	4	7	5	4	6	5	4	23
GA3 std	–	–	2613	87	100	5	2	3	5	3	4	4	4	3	21
					418(M+)	403	390	375	359	347	301	235	207
Lt GA20	22–25	36–39	2450	76	100	12	3	46	9	2	10	3	16
GA20 std	–	–	2451	94	100	13	3	48	11	2	11	5	19
					432(M+)	417	401	373	345	329	313	297	238	207
Lt GA44	26–29	26–28	2778	93	100	13	5	20	3	1	1	1	32	85
GA44 std	–	–	2778	94	100	14	6	21	3	2	1	1	32	74
					462(M+)	434	402	374	345	315	285	258	239	207
Lt GA19	26–29	66–70	2555	95	9	100	25	48	18	11	14	11	24	17
GA19 std	–	–	2558	90	8	100	25	52	20	12	15	12	24	19
					448(M+)	433	416	389	373	357	329	275	251	235	207
Lt GA53	30–35	16–18	2443	91	100	9	16	30	8	5	8	2	21	14	62
					450(M+)	435	418	391	375	359	331	277	253	237	209
[2H2]GA53 std	–	–	2442	–	100	12	18	33	11	6	10	4	26	17	79
					330(M+)	298	286	270	243	228	226	217	211	183	159
Lt GA9	30–35	32–34	2317	68	12	100	7	50	43	5	35	14	8	12	13
GA9 std	–	–	2317	93	16	100	9	56	42	6	39	16	12	17	17

HPLC fractions were selected by comparison with prior separations of standards (std). To determine the Kovats Retention Index (KRI) for GC-MS, all samples were co-injected with Parafilm. Multiple ion monitoring data are presented.

^aFull scans were also obtained and the percentage match with a PC-based GA library is shown (Gaskin and MacMillan, 1991).