Table 2.

Statistical results of the three prognostic biomarker models (F1, F2, and F3)with respect to both treatment response and survival in the Discovery Study (identification of the 34 original subjects) and in the Validation Study (identification of the 20unknown subjects, which were new and different from the 34 original subjects). (A) The ROC AUC value, the 95% confidence interval of the ROC AUC value, the T value and probability of significance of the independent t-Test, the 99.99% confidence interval for the mean score of the R/LTS group and that of the NR/STS group, along with their respective standard deviations, of the F₁, F₂, and F₃ prognostic biomarker models in the Discovery Study are shown. (B) The sensitivity and the specificity of the F₁, F₂, and F₃ prognostic biomarker models in the Discovery Study with respect to both treatment response and survival are shown. (C) The ROC AUC value, the sensitivity, and the specificity of the F₁, F₂, and F₃ prognostic biomarker models in the Validation Study with respect to both treatment response and survival are shown. (D) The T value and probability of significance of the independent t-Test and the mean score of the R/LTS group and that of the NR/STS group, along with their respective standard deviations, of the F₁, F₂, and F₃ prognostic biomarker models in the Validation Study are shown. As can be seen, all six of those group mean scores, as observed in the validation study with the 20 unknown subjects, fall within the 99.99% confidence intervals of the respective group mean scores as predicted in the discovery study (A).

A (Discovery study)
Prognostic test	ROC AUC	95% CI of AUC	T-Value	P (2-tailed)α = 0.001	R/LTS Group [99.99% CI of mean] (SD)	NR/STS Group [99.99% CI of mean] (SD)
F1	0.98929	[0.90449, 0.99884]	−6.7405	1.30 × 10−7	[15.8665, 20.6631] (2.9622)	[22.9236, 27.6717] (3.3651)
F2	0.98929	[0.90321, 0.99886]	−6.7217	1.37 × 10−7	[12.7822, 14.2173] (0.8905)	[14.6982, 15.6030] (0.6407)
F3	0.98214	[0.86165, 0.99782]	−6.4541	2.93 × 10−7	[13.3940, 14.5352] (0.7017)	[14.8956, 15.7599] (0.6082)

B (Discovery study)
Prognostic test	ROC AUC	Sensitivity		Specificity
		Treatment response	Survival	Treatment response	Survival
F1	0.98929	1.0000	0.9500	0.9500	1.0000
F2	0.98929	0.9286	1.0000	1.0000	0.9286
F3	0.98214	1.0000	0.9500	0.9500	1.0000

C (Validation study)
Prognostic test	ROC AUC	Sensitivity		Specificity
		Treatment response	Survival	Treatment response	Survival
F1	1.00000	1.0000	1.0000	1.0000	1.0000
F2	1.00000	1.0000	1.0000	1.0000	1.0000
F3	1.00000	1.0000	1.0000	1.0000	1.0000

D (Validation study)
Prognostic test	T-Value	P (2-tailed) α = 0.001	R/LTS group Mean ± SD	NR/STS group Mean ± SD
F1	−7.8523	3.19 × 10−7	16.1301 ± 2.9288	24.3990 ± 1.5847
F2	−6.8447	2.09 × 10−6	13.0048 ± 0.7932	14.9212 ± 0.3933
F3	−6.2992	6.14 × 10−6	13.6150 ± 0.6979	15.0952 ± 0.2552