Figure 2.

Immunohistochemical staining used in the Choi classification of diffuse large B-cell lymphoma (DLBCL). Sections were first stained for GCET1 protein; if the staining was positive (top left), a parallel section was stained for MUM1 and classified as shown depending on the positivity of MUM1 antibody. If GCET1 staining was negative (bottom left), parallel sections were stained with CD10 as shown. Brown stains indicate positivity and blue hematoxylin counterstains indicate negative expression. Arrowheads show the flow of the data used to generate the algorithm and long arrows the classification reached. GCET, MUM1 lower panel, CD10, BCL6, and FOXP1: bars = 100 µm. MUM1 upper panel and FOXP1 lower panel: bars = 10 µm.