Table 1.
Antibody Dilutions and Positive Controls
| Antibody | Dilution | Positive Control | Manufacturer | Antibody Type | Lot No. |
|---|---|---|---|---|---|
| CLDN-1 | 1:100 | Skin | Invitrogen | Rabbit polyclonal | 624568A |
| CLDN-2 | 1:50 | Colon | Invitrogen | Mouse monoclonal | 787052A |
| CLDN-3 | 1:80 | Colon | Invitrogen | Rabbit polyclonal | 760684A |
| CLDN-4 | 1:200 | Colon | Invitrogen | Mouse monoclonal | 716863A |
| CLDN-7 | 1:100 | Breast | Invitrogen | Rabbit polyclonal | 673366A |
| Ki-67 | 1:100 | Tonsil | DAKO | mouse monoclonal | 00070375 |
| CK-5/6 | 1:2000 | Tonsil | DAKO | Mouse monoclonal | 00033034 |
| CK-20 | 1:600 | Colon adenocarcinoma | DAKO | Mouse monoclonal | 00032659 |
Note: Ki-67 = kinase inhibitor 67; CK-5/6 = cytokeratin-5/6; CK-20 = cytokeratin-20; Invitrogen (Carlsbad, CA); DAKO (Glostrup, Denmark).