Table 1.

CD133 Antibody Clones Used in the Present Study, Including Immunogens and Protein Sizes Reported by Studies and Manufacturers as well as Immunohistochemical Methods, Positive Control Tissues, and Prognostic Data Obtained Using Immunohistochemistry

Antibody Clone	Company	Immunogen	HIER Buffer	Conc.	Detection System	Species and Clonality	Positive Control	Size on Western Blot	Is CD133 Prognostic?
CD133/1 (AC133)	Miltenyi	Second extracellular loop of CD133 (Kemper et al. 2010)	EDTA	1 + 10	PV	Mouse monoclonal	Kidney, pancreas	~120 kDa (Florek et al. 2005; Corbeil et al. 2000)	Yes (Pallini et al. 2008; Thon et al. 2010; Zeppernick et al. 2008)
CD133/1 (W6B3C1)	Miltenyi	Glycosylated epitope 1 on the extracellular domain of CD133	TEG	1 + 40	PV	Mouse monoclonal	Kidney, pancreas, placenta	~120 kDa	No (Christensen et al. 2008)
CD133 (C24B9)	Cell Signaling Technology	A non-glycosylated extracellular region surrounding Asp562 of CD133	EDTA	1 + 50	EnV	Rabbit monoclonal	Kidney, pancreas	~16 kDa (Osmond et al. 2010) ~115 - 130 kDa (Osmond et al. 2010)	NA
CD133 (ab19898)	Abcam	Residue 800 to the C-terminus of CD133	TEG	1 + 400	EnV	Rabbit polyclonal	Kidney, pancreas	~110 kDa	No (Sulman et al. 2008)

TEG, Tris 10 mM, EGTA, 0.5 mM, pH 9; EDTA, 1 mM, pH 8; HIER, heat-induced epitope retrieval; PV, PowerVision+; EnV, EnVision+; NA, not available.