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. 2011 Apr;59(4):366–381. doi: 10.1369/0022155411400866

Figure 2.

Figure 2.

Results of SDS-PAGE analysis of formalin-treated RNase A and myoglobin before and after antigen retrieval (AR). For AR, the formalin-treated proteins (2 mg/ml) were heated at 100C for 20 min followed by heating at 60C for 2 hr in 20 mM Tris-HCl (pH 4.0), 2% (w/v) SDS. (A) Lane M: molecular weight marker; lane 1: native RNase A; lane 2: RNase A treated with formalin for 24 hr at 24C; lane 3: formalin-treated RNase A from lane 2 after AR. (B) Lane M: molecular weight marker; lane 1: native myoglobin; lane 2: myoglobin treated with formalin for 2 hr at 24C; lane 3: the formalin-treated myoglobin from lane 2 after AR. (C) Lane 1: myoglobin treated with formalin for 2 hr at 90C; lane 2: the formalin-treated myoglobin from lane 1 after AR.