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Assessment of protein immunoreactivity to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by Western blotting. (a) Western Blot by anti-GAPDH antibody. Lane 1, RT-Vac + Drierite; lane 2, RT-HC; lane 3, 4C-Vac + Drierite; lane 4, 4C–HC; lane 5, 30C-Vac + Drierite; lane 6, 30C–HC; lane 7, 37C-Vac + Drierite; lane 8, 37C–HC. (b) Quantitative analysis of Western blot (ImageQuant Program version 5.2, Piscataway, NJ). HC, humidity chamber; RT, room temperature; Vac, vacuum packed.
