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Chromogenic detection (NBT/BCIP) of in situ hybridization to miR-138 using DNA + LNA and 2OMe + LNA probes with (500 µg/ml) yRNA or without (0 µg/ml) yRNA in the hybridization buffer. Negative controls (i.e., scrambled and 2–base pair mismatch probe) have been included. Red arrows show Purkinje cells with positive staining (brown). Bar = 200 µm.
