Figure 3.

Double in situ hybridization and fluorescent detection. Probes with different labels were hybridized and detected using TSA deposition of different fluorescent molecules (Cy3 [red] or FITC [green]). Sections were counterstained with DAPI for nuclei visualization in blue. Images were acquired in individual fluorescence channels, before an overlay was produced. This method enables perfect localization of different miRNAs on the same tissue section. In the top row, cells in the mouse cortex show differential expression of miR-124 and miR-130a. The bottom row shows expression of miR-124, predominantly located in neuronal cells of the cerebellum, whereas Purkinje cells show higher expression of miR-138. Bar = 200 µm.