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Genomic Southern analysis. About 10 μg of genomic DNA from cucumber was digested with HincII (lane 1), XbaI (lane 2), NdeI (lane 3), NcoI (lane 4), HindIII (lane 5), and EcoRI (lane 6) and then separated on a 0.6% agarose gel. As a probe, an EcoRI fragment of lox-1 (A) or an EcoRI 3′-end fragment (B) was used. A schematic diagram of lox-1 and templates used for the preparation of probes are shown in C.
