Figure 4.

Accumulation of LOX and icl transcripts during early germinating stages of cucumber cotyledons in the light or dark. Cucumber seeds were germinated on vermiculite and then grown under a 14-h light/10-h dark photoperiod or in complete darkness. A portion of cucumber seedlings grown for 5 d in the dark was transferred to the 14-h light/10-h dark photoperiod. Twenty micrograms of total RNA isolated from each group of cotyledons was separated with a denaturing gel, transferred to a nylon membrane, and sequentially probed with the lox-1 3′ probe (A), the lox-1 internal probe (B), and the icl probe (C). A and B, Some degradation of lox-1 mRNA is evident. Because distinct bands can be seen with the icl probe (as in C), such degradation does not reflect a problem in RNA isolation. D to L, Dark to light transition period.